Department for Molecular Structural Biology

2024



  • Garbers, T., Neumann, P., Wollenhaup,t J., Dickmanns, A., Weiss, MS. and Ficner, R. (2024) Crystallographic fragment screen of the c-di-AMP-synthesizing enzyme CdaA from Bacillus subtilis. Acta Crystallogr F Struct Biol Commun. Sep 1. Online ahead of print.[Abstract] PDB: [8OFH]

  • Neumann, P., Heidemann, JL., Wollenhaupt, J., Dickmanns, A., Agthe, M., Weiss, MS. and Ficner, R. (2024) A small step towards an important goal: fragment screen of the c-di-AMP-synthesizing enzyme CdaA. Acta Crystallogr D Struct Biol. May 1. [Abstract] PDB: [8S47] [8S46] [8S45] [8S49] [8S4A] [8S4C] [8S48] [8S4B]

    • 2023



    • Sievers, K., Neumann, P., Sušac, L., Da Vela, S., Graewert, M., Trowitzsch, S., Svergun, D., Tampé, R. and Ficner, R. (2023) Structural and functional insights into tRNA recognition by human tRNA guanine transglycosylase.. Structure. Dec 27:S0969-2126(23)00447-1[Abstract] PDB: [8OMR]

    • Wicke, D., Neumann, P., Gößringer, M., Chernev, A., Davydov, S., Poehlein, A., Daniel, R., Urlaub, H., Hartmann, RK., Ficner, R. and Stülke, J. (2023) The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro. Nucleic Acids Res. 2023 Dec 5:gkad1171. doi: 10.1093/nar/gkad1171. Online ahead of print. [Abstract]

    • Helm, M., Bohnsack, MT., Carell, T., Dalpke, A., Entian, KD., Ehrenhofer-Murray, A., Ficner, R., Hammann, C., Höbartner, C., Jäschke, A., Jeltsch, A., Kaiser, S., Klassen, R., Leidel, SA., Marx, A., Mörl, M., Meier, JC., Meister, G., Rentmeister, A., Rodnina ,M., Roignant, JY., Schaffrath, R., Stadler, P. and Stafforst, T. (2023) Experience with German Research Consortia in the Field of Chemical Biology of Native Nucleic Acid Modifications. ACS Chem Biol. 2023 Nov 14. doi: 10.1021/acschembio.3c00586. Online ahead of print. [Abstract]

    • Bakti, F., Stupperich, H., Schmitt, K., Valerius, O., Köhler, AM., Meister, C., Strohdiek, A., Harting, R., Sasse, C., Heimel, K., Neumann, P., Ficner, R. and Braus, GH. (2023)Fungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase. Proc Natl Acad Sci U S A. Aug 29;120(35) [Abstract]

    • Garbers, TB., Enders, M., Neumann, P. and Ficner, R. (2023)
      Crystal structure of Prp16 in complex with ADP.
      Acta Crystallogr F Struct Biol Commun. Aug 1;79(Pt 8):200-207       [Abstract]PDB: [8CNT]

    • Ficner, R. (2023) Highlight: integrative structural biology of dynamic macromolecular assemblies. Biol Chem. Aug 8.

    • Enders, M., Neumann, P., Dickmanns, A. and Ficner R. (2023)
      Structure and function of spliceosomal DEAH-box ATPases. Biol Chem. Jul 17. doi: 10.1515/hsz-2023-0157 [Abstract]

    • Warneke, R. , Garbers, TB., Herzberg, C., Aschenbrandt, G., Ficner, R. and Stülke, J. (2023) Ornithine is the central intermediate in the arginine degradative pathway and its regulation in Bacillus subtilis. J Biol Chem. Jun 19:104944. doi: 10.1016 [Abstract]

    • Kehlenbach, RH., Neumann, P., Ficner, R. and Dickmanns, A. (2023)Interaction of nucleoporins with nuclear transport receptors: a structural perspective. Biol Chem May 22. doi: 10.1515/hsz-2023-0155. Online ahead of print. [Abstract]

    • Neumann, P., Kloskowski, P. and Ficner ,R. (2023)Computer-aided design of a cyclic di-AMP synthesizing enzyme CdaA inhibitor microLife, Volume 4, 1-11 [Abstract]PDB:[8C4Q] [8C4O] [8C4N] [8C4R] [8C4M] [8C4J] [8C4P]

    • Enders, M., Ficner, R. and Adio S. (2023) Conformational dynamics of the RNA binding channel regulates loading and translocation of the DEAH-box helicase Prp43. Nucleic Acids Res. May 11:gkad362. doi: 10.1093/nar/gkad362. Online ahead of print. [Abstract]

      • 2022



      • Enders, M., Ficner, R. and Adio, S. (2022) Regulation of the DEAH/RHA helicase Prp43 by the G-patch factor Pfa1. Proc Natl Acad Sci U S A. Nov 29;119(48) [Abstract]

      • Sievers, K. and Ficner, R. (2022) Structure of angiogenin dimer bound to double-stranded RNA. Acta Crystallogr F Struct Biol Commun. Sep 1;78(Pt 9):330-337. [Abstract] PDB:[8AF0]

      • Heidemann, JL., Neumann, P., Krüger, L., Wicke, D., Vinhoven, L., Linden, A,, Dickmanns, A., Stülke, J., Urlaub, H. and Ficner, R. (2022) Structural basis for c-di-AMP-dependent regulation of the bacterial stringent response by receptor protein DarB. J Biol Chem. Jun 14:102144 [Abstract] PDB: [6YJ7] [6YJ8] [6YJ9] [6YJA]


        • 2021



        • Perera, RP., Shaikhqasem, A., Rostam, N., Dickmanns, A., Ficner, R., Tittmann, K. and Dosch, R. (2021) Bucky Ball Is a Novel Zebrafish Vasa ATPase Activator. Biomolecules. Oct 13;11(10):1507. [Abstract]

        • Gomkale, R., Linden, A., Neumann, P., Schendzielorz, AB., Stoldt, S., Dybkov, O., Kilisch, M., Schulz, C., Cruz-Zaragoza, LD., Schwappach, B., Ficner, R., Jakobs, S., Urlaub, H. and Rehling, P. (2021) Mapping protein interactions in the active TOM-TIM23 supercomplex. . Nat Commun. Sep 29;12(1):5715 [Abstract]

        • Sievers, K., Welp, L., Urlaub, H. and Ficner, R. (2021) Structural and functional insights into human tRNA guanine transgylcosylase. RNA Biol. 2021 Jul 9. doi: 10.1080/15476286.2021.1950980. Online ahead of print. [Abstract] PDB: [7NQ4]

        • Blersch, KF., Burchert, JP., August, SC., Welp, L., Neumann, P., Köster, S., Urlaub, H. and Ficner R. (2021) Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA. RNA Biol. 2021 May 19:1-14. [Abstract] PDB: [7NYB] [7NZI] [7NZJ]

        • Bohnsack, KE., Ficner ,R., Bohnsack, MT. and Jonas, S. (2021) Regulation of DEAH-box RNA helicases by G-patch proteins. Biol Chem. Jan 6;402(5):561-579 [Abstract]

        • Hamann, F., Zimmerningkat, LC., Becker, RA., Garbers, TB., Neumann, P., Hub, JS. and Ficner,R. (2021) The structure of Prp2 bound to RNA and ADP-BeF3- reveals structural features important for RNA unwinding by DEAH-box ATPases. Acta Crystallogr D Struct Biol. Apr 1;77(Pt 4):496-509. [Abstract] PDB: [6ZM2]

        • Shaikhqasem, A., Schmitt, K., Valerius, O. and Ficner, R. (2021) Crystal structure of human CRM1, covalently modified by 2-mercaptoethanol on Cys528, in complex with RanGTP. Acta Crystallogr F Struct Biol Commun. Mar 1;77(Pt 3):70-78 [Abstract] PDB: [7B51]

        • Krüger, L., Herzberg, C., Wicke, D., Bähre, H., Heidemann, JL., Dickmanns, A., Schmitt, K., Ficner, R. and Stülke, J. (2021)A meet-up of two second messengers: the c-di-AMP receptor DarB controls (p)ppGpp synthesis in Bacillus subtilis. Nat Commun. Feb 22;12(1):1210. doi: 10.1038/s41467-021-21306-0. [Abstract]

        • Valpadashi. A., Callegari, S., Linden, A., Neumann, P., Ficner, R. ,Urlaub, H., Deckers, M. and Rehling.P. (2021)Defining the architecture of the human TIM22 complex by chemical crosslinking. P. FEBS Lett. Jan;595(2):157-168 [Abstract]

          • 2020



          • Netz, E., Dijkstra, TMH., Sachsenberg, T., Zimmermann, L., Walzer, M., Monecke, T., Ficner, R., Dybkov, O., Urlaub, H. and Kohlbacher, O. (2020) OpenPepXL: An open-source tool for sensitive identification of cross-linked peptides in XL-MS. Mol Cell Proteomics Oct 16:mcp.TIR120.002186. doi: 10.1074/mcp.TIR120.002186. Online ahead of print. PMID: 33067342 [Abstract]

          • Metje-Sprink, J., Groffmann, J., Neumann, P., Barg-Kues, B., Ficner, R., Kühnel, K., Schalk, AM. And Binotti B. (2020)Crystal structure of the Rab33B/Atg16L1 effector complex. Sci Rep. 2020 Jul 31;10(1):12956. doi: 10.1038/s41598-020-69637-0. [Abstract]PDB: [6SUR]

          • Shaikhqasem, A., Dickmanns, A., Neumann, P. and Ficner R. (2020)Characterization of inhibition reveals distinctive properties for human and Saccharomyces cerevisiae CRM1. J Med Chem. Jul23;63 (14):7545-7558. [ Abstract]PDB:[6TVO]

          • Munzel, L., Neumann, P., Otto, FB., Krick, R., Metje-Sprink, J., Kroppen, B., Karedla, N., Enderlein, J., Meinecke, M., Ficner, R., and Thumm. M. (2020) Atg21 organizes Atg8 lipidation at the contact of the vacuole with the phagophore. Autophagy. 2020 Jun 9:1-21. doi: 10.1080/15548627.2020.1766332. Online ahead of print. PMID: 32515645 [Abstract] PDB:[6RGO]

          • Linden, A., Deckers, M., Parfentev, I., Pflanz, R., Homberg, B., Neumann, P., Ficner, R., Rehling, P. and Urlaub, H. (2020) A cross-linking mass spectrometry approach defines protein interactions in yeast mitochondria. Mol Cell Proteomics. Jul; 19(7):1161-1178. [Abstract]

          • Gibhardt,J., Heidemann, JL., Bremenkamp, R., Rosenberg, J., Seifert, R., Kaever, V., Ficner, R. and Commichau, FM. (2020) An extracytoplasmic protein and a moonlighting enzyme modulate synthesis of c-di-AMP in Listeria monocytogenes. Environ Microbiol. Jul;22(7):2771-2791 [Abstract]

          • Hamann, F., Schmitt, A., Favretto, F., Hofele, R., Neumann, P., Xiang, S., Urlaub, H., Zweckstetter, M. and Ficner R. (2020)Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2. Proc Natl Acad Sci U S A. Feb 11; 117 (6): 2948-2956 [Abstract] PDB:[6RMC][6RMB][6RMA][6RM9][6RM8] ]

            • 2019



            • Heidemann, JL., Neumann, P., Dickmanns, A. and Ficner.R. (2019) Crystal structures of the c-di-AMP synthesizing enzyme CdaA. J Biol Chem. Jul 5; 294 (27) 10463 - 10470[Abstract] PDB: [6HVL] [6HVM] [6HVN]

            • Hamann, F., Enders, M.and Ficner R. (2019) Structural basis for RNA translocation by DEAH-box ATPases. Nucleic Acids Res.May 7; 47 (8) 4349 - 4362 [Abstract] PDB: [6I3O][6I3P][6QIC][6QID][6QIE]

              • 2018



              • Bonnin, E., Cabochette, P., Filosa, A., Jühlen, R., Komatsuzaki, S., Hezwani, M., Dickmanns, A., Martinelli, V., Vermeersch, M., Supply, L., Martins, N., Pirenne, L., Ravenscroft, G., Lombard, M., Port, S., Spillner, C., Janssens, S., Roets, E., Van Dorpe, J., Lammens, M., Kehlenbach, RH., Ficner, R., Laing, NG., Hoffmann, K., Vanhollebeke, B. and Fahrenkrog, B. (2018)Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence. PLoS Genet. Dec 13;14(12):e1007845. doi: 10.1371/journal.pgen.1007845. [Epub ahead of print] [Abstract]

              • Commichau, FM., Heidemann, JL., Ficner, R. and Stülke, J. (2018) Making and breaking of an essential poison: the cyclases and phosphodiesterases that produce and degrade the essential second messenger cyclic di-AMP in bacteria. J Bacteriol. 2018 Sep 17. pii: JB.00462-18. doi: 10.1128/JB.00462-18. [Epub ahead of print] Review [Abstract]

              • Johannsson, S., Neumann, P. and Ficner R. (2018) Crystal Structure of the Human tRNA Guanine Transglycosylase Catalytic Subunit QTRT1. Biomolecules Aug 24:8 (3) [Abstract]PDB:[6H42][6H45]

              • Schmitt, A., Hamann, F., Neumann, P. and Ficner R. (2018) Crystal structure of the spliceosomal DEAH-box ATPase Prp2. Acta Crystallogr D Struct Biol. Jul 1;74(Pt 7):643-654 [Abstract] PDB:[6FA9][6FAA][6FAC][6FA5]

              • Johannsson, S., Neumann, P., Wulf, A., Welp, LM., Gerber, HD., Krull, M., Diederichsen, U., Urlaub, H. and Ficner R. (2018 )Structural insights into the stimulation of S. pombe Dnmt2 catalytic efficiency by the tRNA nucleoside queuosine. Sci Rep. Jun 11;8(1):8880. doi: 10.1038/s41598-018-27118-5. [Abstract] PDB:[6FDF]

              • Neumann, P., Dickmanns, A. and Ficner R. (2018) Validating Resolution Revolution. Structure. May 1;26(5) 785-795 [Abstract][Erratum]

              • Dickmanns, A. Zschiedrich, CP., Arens, J., Parfentev, I., Gundlach, J., Hofele, R., Neumann, P., Urlaub, H., Görke, B., Ficner, R. and Stülke J. (2018). Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh in Bacillus subtilis. J Biol Chem. 2018 Apr 30; 29(16) 5781 - 5792 [Abstract] PDB:[6F2C]

              • Kolog Gulko, M., Heinrich, G., Gross, C., Popova, B., Valerius, O., Neumann, P., Ficner, R. and Braus, GH. (2018). Sem1 links proteasome stability and specificity to multicellular development. PLoS Genet. Feb 5;14(2) :e1007141. doi: 10.1371/journal.pgen.1007141. [Epub ahead of print] [Abstract]


                • 2017



                • Bujacz, A., Talaj, JA., Zielinski, K., Pietrzyk-Brzezinska, AJ., Neumann P. (2017). Crystal structures of serum albumins from domesticated ruminants and their complexes with 3,5-diiodosalicylic acid. Acta Crystallogr D Struct Biol. Nov 1;73(Pt 11):896-909. [Abstract]PDB:[5ORI] [5ORF] [5OSW][5OTB]

                • Meese S, Cepeda AP, Gahlen F, Adams CM, Ficner R, Ricci AJ, Heller S, Reisinger E, Herget M. (2017). Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Front Synaptic Neurosci. 9:13. [Abstract]

                • Gonzalez GM, Durica-Mitic S, Hardwick SW, Moncrieffe MC, Resch M, Neumann P, Ficner R, Görke B, Luisi BF. (2017). Structural insights into RapZ-mediated regulation of bacterial amino-sugar metabolism. Nucleic Acids Res., 45:10845-10860 [Abstract] PDB: [5O5O] [5O5Q] [5O5S]

                • Ficner, R., Dickmanns, A., Neumann, P. (2017). Studying Structure and Function of Spliceosomal Helicases. Methods, 125:63-69. [Abstract]

                • Newie J, Neumann P, Werner M, Mata RA, Ficner R, Feussner I. (2017). Lipoxygenase 2 from Cyanothece sp. controls dioxygen insertion by steric shielding and substrate fixation. Sci Rep. 7:2069. [Abstract] PDB: [5MED] [5MEE] [5MEF] [5MEG]

                • Tauchert MJ, Fourmann JB, Lührmann R, Ficner R. (2017). Structural insights into the mechanism of the DEAH-box RNA helicase Prp43. Elife. 6:e21510. [Abstract] PDB: [5LTA] [5LTJ] [5LTK]

                • Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer-Pretz V, Braus GH, Valerius O. (2017). Asc1p/RACK1 connects ribosomes to eukaryotic phospho-signaling. Mol. Cell. Biol., 37:e000279-16. [Abstract]

                • Fourmann JB, Tauchert MJ, Ficner R, Fabrizio P, Lührmann R. (2017) Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2. Nucleic Acids Res., 45:4068 - 4080. [Abstract]


                  • 2016




                  • Fischer, N., Neumann, P., Bock, LV., Maracci, C., Wang, Z., Paleskava, A., Konevega, AL., Schröder, GF., Grubmüller, H., Ficner, R., Rodnina, MV. and Stark, H. (2016) The pathway to GTPase activation of elongation factor SelB on the ribosome. Nature, 540: 80-85. [Abstract]

                  • Boesler, C., Rigo, N., Anokhina, MM., Tauchert, MJ., Agafonov DE., Kastner, B., Urlaub, H., Ficner, R., Will, CL. and Lührmann, R. (2016) A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity. Nat Commun. 7:11997. [Abstract]

                  • Tauchert, MJ., Hémonnot, C., Neumann, P., Köster, S., Ficner, R. and Dickmanns A. (2016) Impact of the crystallization condition on importin-? conformation. Acta Crystallogr D Struct Biol. 72:705-717. [Abstract] PDB: [4XRI] [4XRK]

                  • Tauchert, MJ. and Ficner, R. (2016) Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum. Acta Crystallogr F Struct Biol Commun. 72:409-16. [Abstract] PDB: [5DTU]

                  • Fourmann, JB., Dybkov, O., Agafonov, DE., Tauchert, MJ., Ficner, R., Fabrizio, P. and Lührmann, R. (2016) The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction. Elife. Apr 26;5 [Abstract]

                  • Mahal, K., Biersack, B., Schruefer, S., Resch, M.,Ficner, R., Schober, R. and Mueller, T. (2016) Combretastatin A-4 derived 5-(1-methyl-4-phenyl-imidazol-5-yl)indoles with superior cytotoxic and anti-vascular effects on chemoresistant cancer cells and tumors. Eur J Med Chem. 118:9-20. [Abstract]

                  • Koch, C., Neumann, P., Valerius, O., Feussner, I. and Ficner, R. (2016) Crystal Structure of Alcohol Oxidase from Pichia pastoris. PLoS One Feb 23;11(2) [Abstract] PDB: [5HSA]

                  • Tauchert, MJ., Fourmann, JB., Christian, H., Lührmann, R. and Ficner R. (2016) Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum. Acta Crystallogr F Struct Biol Commun. 72:112-20. [Abstract] PDB: [5D0U]

                  • Newie, J., Andreou, A., Neumann, P., Einsle, O., Feussner, I. and Ficner, R. (2016) Crystal structure of a lipoxygenase from Cyanothece sp. may reveal novel features for substrate acquisition. J Lipid Res. 57: 276-87. [Abstract] PDB: [5EK8]


                    • 2015





                    • Monecke, T. , Dickmanns, A., Weiss, MS., Port, SA., Kehlenbach, RH. and Ficner R. (2015) Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1-RanGTP-SPN1-Nup214 quaternary nuclear export complex. Acta Crystallogr F Struct Biol Commun. Dec 1;71(Pt 12):1481-1487. [Abstract] PDB: [5DIS]

                    • Dickmanns, A. , Kehlenbach, RH. and Fahrenkrog B. (2015) Nuclear Pore Complexes and Nucleocytoplasmic Transport: From Structure to Function to Disease. Int Rev Cell Mol Biol. 320:171-233. [Abstract]

                    • Port, SA., Monecke, T., Dickmanns, A., Spillner, C., Hofele, R., Urlaub, H., Ficner, R. and Kehlenbach, RH. (2015)Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export. Cell Rep. Oct 27; 13(4): 690-702 [Abstract] PDB: [5DIS]


                    • Wechsler, C., Meyer, D., Loschonsky, S., Funk, LM., Neumann, P., Ficner, R., Brodhun, F., Müller, M. and Tittmann, K. (2015)Tuning and switching enantioselectivity of asymmetric carboligation in an enzyme through mutational analysis of a single hot spot. Chembiochem., Dec: 16(18)2580-4 [Abstract]PDB: [4ZP1]


                    • Jung, S., Maritzen, T., Wichmann, C., Jing, Z., Neef, A., Revelo, NH., Al-Moyed, H., Meese, S., Wojcik, SM., Panou, I., Bulut, H., Schu, P., Ficner, R., Reisinger, E., Rizzoli, SO., Neef, J., Strenzke, N., Haucke, V. and Moser. T. (2015) Disruption of adaptor protein 2? (AP-2?) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing. EMBO J. , Nov 3; 34(21): 2686 - 702 [Abstract]

                    • Dickmanns, A., Monecke, T. and Ficner, R. (2015) Structural Basis of Targeting the Exportin CRM1 in Cancer. Cells, 4(3), 538 - 568 Review. [Abstract]

                    • Kuhle, B. Eulig, NK. and Ficner R. (2015) Architecture of the eIF2B regulatory subcomplex and its implications for the regulation of guanine nucleotide exchange on eIF2. Nucleic Acids Res. Nov 16; 43 (20): 9994 - 10014 [Abstract]; PDB:[4ZEM];[4ZEO];[5DBO]


                    • Mahal, K., Ahmad, A., Sethi, S., Resch, M., Ficner, R., Sarkar, FH. , Schobert,R. and Biersack, B. (2015). Role of JNK and NF-?B in mediating the effect of combretastatin A-4 and brimamin on endothelial and carcinoma cells. Cell Oncol (Dordr).Dec; 38(6): 463 - 78. [Abstract]


                    • Halder K., Dölker N., Van Q., Gregor I., Dickmanns A., Baade I., Kehlenbach RH., Ficner R., Enderlein J., Grubmüller H. and Neumann H.(2015).MD Simulations and FRET Reveal an Environment-Sensitive Conformational Plasticity of Importin-ß. Biophys 109(2):277-86.[Abstract]


                    • Melin, J., Kilisch, M., Neumann, P., Lytovchenko, O., Gomkale, R.,Schendzielorz, A., Schmidt, B., Liepold, T., Ficner, R., Jahn, O., Rehling, P. and Schulz, C. (2015). A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochim Biophys Acta. May 7; 1853(8): 1850 - 1859 [Abstract]


                    • Commichau,FM., Dickmanns, A., Gundlach, J., Ficner, R. and Stülke, J. (2015).A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.Mol Microbiol. 97(2):189-204. [Abstract]


                    • Fischer, N., Neumann, P., Konevega, A.L., Bock, L.V., Ficner, R., Rodnina, M.V. and Stark, H. (2015). Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM. Nature, Apr 23;520(7548):567-70 [Abstract]


                    • Rosenberg, J., Dickmanns,A., Neumann, P., Gunka, K., Arens, J., Kaever, V., Stülke, J., Ficner, R., and Commichau, F.M.(2015). Structural and biochemical analysis of the essential diadenylate cyclase CdaA from Listeria monocytogenes. J. Biol. Chem., Mar 6;290(10):6596-606. [Abstract]; PDB: [4RV7]


                    • Gundlach, J., Dickmanns A., Schröder-Tittmann K., Neumann P., Kaesler J., Kampf J., Herzberg C., Hammer E., Schwede F., Kaever V., Tittmann K., Stülke J., and Ficner R. (2015). Identification, characterization and structure analysis of the c-di-AMP binding PII-like signal transduction protein DarA. J. Biol. Chem., Jan 30; 290 (5): 3069-80 [Abstract]; PDB: [4RLE]





                      • 2014




                      • Neumann, P. and Tittmann, K. (2014). Marvels of enzyme catalysis at true atomic resolution: distortions, bond elongations, hidden flips, protonation states and atom identities. Curr Opin Struct Biol. Dec;29:122-33. [Abstract]

                      • Kuhle, B. and Ficner, R. (2014). Structural insight into the recognition of amino-acylated initiator tRNA by eIF5B in the 80S initiation complex. BMC Struct. Biol. Sep 17;14(1):20. [Abstract]

                      • Kuhle, B. and Ficner, R. (2014). A monovalent cation acts as structural and catalytic cofactor in translational GTPases. EMBO J. Sep 15. pii: e201488517. [Abstract]; PDB: [4TMT]; [4TMV]; [4TMX]; [4TMW]; [4TMZ]; [4TN1]

                      • Möhlmann, S., Mathew, R., Neumann, P., Schmitt, A., Lührmann, R. and Ficner, R. (2014). Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28. Acta Crystallogr. D70: 1622-1630. [Abstract] ; PDB: [4NHO]

                      • Monecke, T., Dickmanns, A. and Ficner R. (2014) Allosteric control of the exportin CRM1 unraveled by crystal structure analysis. FEBS J., Sep;281(18):4179-94. [Abstract]

                      • Liu Y, Neumann P, Kuhle B, Monecke T, Schell S, Chari A, Ficner R. (2014). Translation Initiation Factor eIF3b Contains a Nine-Bladed b-Propeller and Interacts with the 40S Ribosomal Subunit. Structure, 22:1-8. [Abstract]; PDB: [4NOX]

                      • Neumann P, Lakomek K, Naumann PT, Erwin WM, Lauhon CT. and Ficner R. (2014). Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification. Nucleic Acids Res., 42:6673-6685. [Abstract] [Article] ; PDB: [4KR6] [4KR7] [4KR9]

                      • Kuhle, B. and Ficner, R. (2014). eIF5B employs a novel domain release mechanism to catalyze ribosomal subunit joining. EMBO Journal, 10:1177-91 [Abstract]; PDB: [4NCF] [4NCL] [4NCN] [4NG3] [4N3N] [4N3S]

                      • Mahal, K., Resch,M., Ficner, R., Schobert, R., Biersack, B. and Mueller, T. (2014). Effects of the Tumor-Vasculature-Disrupting Agent Verubulin and Two Heteroaryl Analogues on Cancer Cells, Endothelial Cells, and Blood Vessels. ChemMedChem, 4:847-54 [Abstract]

                      • Ahmed, J.L. and Ficner, R. 2014. RNA synthesis and purification for structural studies. RNA Biol, 11:5 [Abstract]

                      • Monecke T, Buschmann J, Neumann P, Wahle E. and Ficner R. (2014). Crystal Structures of the Novel Cytosolic 5'-Nucleotidase IIIB Explain Its Preference for m7GMP. PLoS One, 9:e90915. [Abstract]; [Article]; PDB: [4NV0] [4NWI]

                      • Lehneck, R., Neumann, P., Vullo, D., Elleuche, S., Supuran, C.T., Ficner, R. and Pöggeler, S. (2014). Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora. FEBS J., 7:1759-72. [Abstract] ; PDB: [4O1K] [4O1J]

                      • Khoshnevis, S.,L., Gunisová, S., Vlcková, V., Kouba, T., Neumann, P., Beznosková, P., Ficner, R. and Valasek, L.S. (2014). Structural integrity of the PCI domain of eIF3a/TIF32 is critically required for mRNA recruitment to the 43S pre-initiation complexes. Nucleic Acids Res., 42: 4123-4139. [Abstract];[Article]; PDB: [4K51]

                      • Christian, H., Hofele, R., Urlaub, H. and Ficner , R. (2014). Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry. Nucl. Acid Res., 42:1162-79 [Abstract]



                      2013



                      • Ahmed, Y.L., Gerke, J., Park, H.-S., Bayram, Ö., Neumann, P., Min Ni, M., Dickmanns, A., Kim, S.C. , Yu, J.-H., Braus, G.H. and Ficner, R. (2013). The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-kB. PlosBiol., 11(12): e1001750 [Abstract] [Article] ; PDB: [4N6Q] [4N6R].

                        Roberts RG (2013). The Velvet Underground Emerges. PLoS Biol 11(12): e1001751. [Synopsis to Ahmed et al. in PloS Biol]

                      • Xiang, S.Q. , Gapsys, V., Kim, H.-Y., Bessonov, S., Hsiao,H.-H., Möhlmann, S., Klaukien, K., Ficner, R., Becker, S., Urlaub, H., Lührmann, R., de Groot, B. and Zweckstetter, M. (2013). Phosphorylation drives a dynamic switch in serine/arginine-rich proteins. Structure, 12:2162-74 [Abstract]

                      • Lüdtke, S., Neumann, P., Erixon, K.M., Kluger, R., Ficner, R. and Tittmann, K. (2013). Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate. Nat. Chem., 5:762-767 [Abstract]; PDB: [4KXV] [4KXW] [4KXU] [4KXX] [4KXY]

                      • Dölker, N., Blanchet, C.E., Voß, B., Haselbach, D., Kappel , C., Monecke, T., Svergun, D.I., Stark, H., Ficner, R., Zachariae, U., Grubmüller, H. and Dickmanns, A. (2013) . Structural determinants and mechanism of mammalian CRM1 allostery. Structure, 21:1350-1360. [Abstract]; EMDB: [EMD-2274] [EMD-5564]

                      • Meyer, D., Neumann, P., Ficner, R. and Tittmann, K. (2013). Observation of a stable carbene at the active site of an enzyme. Nat. Chem. Biol., 9:488-490.[Abstract]; PDB: [4KGD]

                      • Naumann, P.-T., Lauhon, C.T. and Ficner, R. (2013). Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase - RNA complex. Acta Cryst. F69: 421-424. [Abstract]

                      • Volkov, A., Khoshnevis, S., Neumann, P., Herrfurth, C., Wohlwend, D., Ficner, R. and Feussner, I. (2013). Crystal structure analysis of a fatty acid double bond hydratase from Lactobacillus acidophilus. Acta Crystallogr. D69: 648-657 [Abstract]; [4IA5][4IA6]

                      • Fourmann, J.B., Schmitzová, J., Christian, H., Urlaub, H., Ficner, R., Boon, K.-L., Fabrizio, P., and Lührmann, R. (2013). Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system. Genes&Dev. 27: 413-428. [Abstract]

                      • Resch, M., Göpel, Y., Görke, B. and Ficner R. (2013). Crystallization and preliminary X-ray diffraction analysis of YhbJ from Escherichia coli, a key protein involved in the GlmYZ sRNA regulatory cascade. Acta Cryst. F69: 109-114. [Abstract]

                      • Monecke, T., Haselbach, D., Voß, B., Russek, A., Neumann, P., Thomson, E., Hurt, E., Zachariae, U., Stark, H., Grubmüller, H., Dickmanns, A. and Ficner, R. (2013). Structural basis for cooperativity of CRM1 export complex formation. PNAS USA. 110: 960-965. [Abstract]; [Publication]; PDB: [4FGV], [4HZK], EMDB: [EMD-2110], [EMD-2111]



                      2012



                      • Khoshnevis, S., Hauer, F., Milón, P., Stark, H., and Ficner, R. (2012). Novel insights into the architecture and protein interaction network of yeast eIF3. RNA 18: 2036-2319.[Abstract]

                      • Neumann, P., Brodhun, F., Sauer, K., Herrfurth, C., Hamberg, M., Brinkmann, J., Scholz, J., Dickmanns, A., Feussner, I. and Ficner R. (2012). Crystal structures of Physcomitrella patens AOC1 and 2: Insights into the enzyme mechanism and differences in substrate specificity. Plant Physiol. 160: 251-1266. [Abstract]; PDB: [4H69], [4H6A], [4H6B], [4H6C]

                      • Kudlinzki, D., Schmitt, A., Christian, H. and Ficner, R. (2012) Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22. Biol. Chem. 393: 1131?1140. [Abstract]; PDB: [3I4U]

                      • Bayram, Ö., Ahmed, Y.L., Ficner, R. and Braus G.H. (2012). The A. nidulans MAPK module AnSte11-Ste50-Ste7-Fus3 controls development and secondary metabolism. PLoS Genetics. 8(7): e1002816. [Abstract]; [Publication]

                      • Meyer D., Neumann P., Koers E., Sjuts H., Lüdtke S., Sheldrick G.M., Ficner R., Tittmann K. (2012). Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin. PNAS USA.109: 10867-72. [Abstract]; PDB: [4FEG] , [4FEE]

                      • Schulz, E.-C., Roth H.M., Ankri, S. and Ficner, R. (2012).
                        Structure Analysis of Entamoeba histolytica DNMT2 (EhMeth). PlosONE.7(6):e38728 [Abstract]; [Publication]; PDB: [3QV2]



                      2011



                      • Schulz C., Lytovchenko O., Melin J., Chacinska A., Guiard B., Neumann P., Ficner R., Jahn O., Schmidt B., Rehling P. (2011). Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex.
                        J Cell Biol. 195(4):643-56. [Abstract]

                      • Lehwess-Litzmann A., Neumann P., Parthier C., Lüdtke S., Golbik R., Ficner R. & Tittmann K. (2011). Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism. Nat Chem Biol. 7(10):678-84. [Abstract]; PDB: [3S0C][3S1U][3S1V][3S1W][3S1X]

                      • Schulz E.-C., Tietzel M., Tovy A., Ankrib S., Ficner R. (2011). Structure analysis of Entamoeba histolytica enolase. Acta Cryst. D67: 619?-627. [Abstract]; PDB: [3QTP]

                      • Schulz E.-C., Bergfeld, A.K., Ficner R. and Mühlenhoff M. (2011). Structural basis for capsule form-variation in Escherichia coli K1: the crystal structure of polySia specific O-acetyltransferase NeuO. PLoS One. 6(3):e17403. [Abstract]; PDB: [3JQY]

                      • Schulz E.-C. and Ficner R. (2011). Knitting and snipping: chaperones in β-helix folding. Curr Opin Struct Biol. (2):232-9. [Abstract]

                      • Helfmann S., Neumann P., Tittmann K., Moser T., Ficner R., Reisinger E. (2011). The Crystal Structure of the C(2)A Domain of Otoferlin Reveals an Unconventional Top Loop Region. J Mol Biol. 406(3):479-90. [Abstract]; PDB: [3L9B]

                      • Dickmanns A., Damerow S., Neumann P.; Schulz E.-C., Lamerz A.-C., Routier H. F., Ficner R. (2011). Structural basis for the broad substrate range of the UDP-sugar pyrophosphorylase from Leishmania major. J.Mol.Biol. 405(2):461-78. [Abstract]; PDB: [3OGZ][3OH0][3OH1][3OH2][3OH3][3OH4]



                      2010



                      • Güttler T., Madl T., Neumann P., Deichsel D., Corsini L., Monecke T., Ficner R., Sattler M. & Görlich D. (2010). NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1. Nature Struct. Mol. Biol. (11):1367-76. [Abstract]; PDB: [3NBY][3NBZ][3NC0][3NC1]

                      • Khoshnevis S., Neumann P., Ficner, R. (2010). Crystal Structure of the RNA Recognition Motif of Yeast Translation Initiation Factor eIF3b Reveals Differences to Human eIF3b. PLoS ONE 5(9): e12784. [Abstract]; PDB: [3NS6][3NS5]

                      • Lakomek, K., Dickmanns, A., Ciirdaeva, E., Schomacher, L. and Ficner, R. (2010). Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA. J Mol Biol. 18;399(4):604-17. [Abstract]; PDB: [3G00][3G0A][3G0R][3G1K][3G2C][3G2D][3G38][3G3C][3G3Y][3G4T][3G8V][3G91][3FZI][3GA6]

                      • Kühn-Hölsken, E., Lenz, C., Dickmanns, A., Hsiao, H.-H., Richter, F.M., Kastner, B., Ficner, R., and Urlaub, H. (2010). Mapping the binding site of Snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Res. 38(16):5581-93. [Abstract]

                      • Schulz, E.-C., Schwarzer, D, Frank, M., Stummeyer, K., Mühlenhoff, M., Dickmanns, A., Gerardy-Schahn, R., and Ficner, R. (2010). Structural basis for recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein endoNF. J. Mol. Biol., 937: 341-51. [Abstract]; PDB: [3GVJ][3GVK][3GVL]

                      • Schulz, E.-C., Dickmanns, A., Urlaub, H., Schmitt, A., Mühlenhoff, M., Stummeyer, K., Schwarzer, D., Gerardy-Schahn, R., and Ficner, R. (2010). Crystal structure of a novel intramolecular chaperone mediating triple ß-helix folding. Nature Struct. Mol. Biol., 17:210-5. [Abstract]; PDB: [3GUD][3GW6]

                      • Schulz, E.-C., Neumann, P., Gerardy-Schahn, R., Sheldrick, G.M., and Ficner, R. (2010). Crystal structure analysis of endosialidaseNF at 0.98 Å resolution. Acta Crystallogr. D66: 176-80. [Abstract]; PDB: [3JU4]

                      • Khoshnevis, S., Gross, T., Rotte, C., Baierlein, C., Ficner, R., and Krebber, H. (2010). The iron-sulfur protein Rli1 functions in translation termination. EMBO Rep., 11: 214- 9 [Abstract]



                      2009




                      • Warkocki, Z., Odenwälder, C., Schmitzova, J., Platzmann, F., Stark, H., Urlaub, H., Ficner, R, Fabrizio, P. and Lührmann, R. (2009). Reconstitution of both Steps of Saccharomyces cereviseae splicing with purified spliceosomal components. Nature Struct. Mol. Biol. 12: 1237-43. [Abstract]

                      • Kudlinzki, D., Nagel, C., and Ficner, R. (2009). Crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of the human spliceosomal DExD/H-box protein hPrp22.Acta Crystallogr. F 65: 956-958. [Abstract]

                      • Lakomek, K., Dickmanns, A., Kettwig, M., Urlaub, H., Ficner, R., and Luebke, T. (2009). Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography. BMC Struct Biol.,9: 56. [Abstract]; PDB: [3FGR][3FGT][3FGW]

                      • Oschlies, M., Dickmanns, A., Haselhorst, T., Schaper, W., Stummeyer, K., Tiralongo, J., Weinhold, B., Gerardy-Schahn, R., von Itzstein, M., Ficner, R., and Münster-Kühnel, A.K. (2009). A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme. J. Mol. Biol. 393:83-97. [Abstract]; PDB: [3EWI]

                      • Goette, M., Stumpe, M.C., Ficner, R. and Grubmüller, H. (2009). Molecular determinants of snurportin1 ligand affinity and structural response upon binding. Biophys. J. 97: 581-589. [Abstract] PDB: [1XK5]

                      • Monecke, T., Dickmanns, A., and Ficner, R. (2009). Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1. Nucleic Acids Res. 37: 3865-3877. [Abstract]; PDB: [3GDH]

                      • Ficner, R. (2009). Novel structural insights into class I and II histone deacetylases. Curr. Top. Med. Chem. 9: 235-240. [Abstract]

                      • Monecke, T., Güttler, T., Neumann, P., Dickmanns, A., Görlich, D., and Ficner, R. (2009). Crystal structure of the nuclear export receptor CRM1 in complex with snurportin1 and RanGTP. Science, 324:1087-1091. [Abstract]; PDB: [3GJX]

                      • Monecke,T., Dickmanns, A., Strasser A., and Ficner R. (2009). Structure Analysis of the conserved Methyltransferase Domain of Human Trimethylguanosine Synthase TGS1. Acta Crystallogr. D65: 332-338. [Abstract]; PDB: [3EGI]

                      • Lakomek, K., Dickmanns, A., Müller, U., Kollmann, K., Deuschl, F., Berndt, A., Lübke, T., and Ficner, R. (2009). De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse. Acta Crystallogr. D 65: 220-8. [Abstract]; PDB: [3FBXI]

                      • Padmanabhan, N., Fichtner, L., Dickmanns, A., Ficner, R., Schulz, J.B., and Braus, G. (2009). The Yeast HtrA Orthologue Ynm3 Is a Protease with Chaperone Activity that Aids Survival under Heat Stress.Mol.Cell. Biol., 20: 68-77. [Abstract]



                      2008




                      • Monecke,T., Schell, S., Dickmanns, A., and Ficner R. (2008). Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m7G-cap binding mode. J. Mol. Biol. 382: 827-34. [Abstract]; PDB: [3CTR]

                      • Mathew, R., Hartmuth, K., Möhlmann, S., Urlaub, H., Ficner, R., and Lührmann, R. (2008). Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome. Nature Struct. Mol. Biol. 15: 435-443. [Abstract]

                      • Schäfer, S., Saunders, L., Eliseeva, E., Velena, A., Jung, M., Schwienhorst, A., Strasser, A., Dickmanns, A., Ficner, R., Schlimme, S., Sippl, W., Verdin, E., and Jung, M. (2008). Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs). Bioorg. Med. Chem. 16: 2011-2033. [Abstract]; PDB: [2VGC]



                      2007




                      • Wohlwend, D., Strasser, A., Dickmanns, A., and Ficner R. (2007). Structural Basis for RanGTP Independent Entry of Spliceosomal U snRNPs into the Nucleus. J. Mol. Biol. 374: 1129-1138. [Abstract]; PDB: [2QNA]

                      • Nielsen, T.K., Liu, S., Lührmann, R. and Ficner, R. (2007). Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2). J. Mol. Biol. 369: 902-908. [Abstract]; PDB: [1SYX]

                      • Nielsen, T.K., Hildmann, C., Riester, D., Wegener, D., Schwienhorst, A. and Ficner, R. (2007). Complex structure of a bacterial class 2 histone deacetylase homologue with a trifluoromethylketone inhibitor. Acta Crystallogr. F63: 270-273. [Abstract]; PDB: [2GH6]

                      • Wohlwend, D., Strasser, A., Dickmanns, A., Doenecke, D. and Ficner R. (2007). Thermodynamic analysis of H1 nuclear import: receptor tuning of importinbeta/importin7. J. Biol. Chem. 282: 10707-10719. [Abstract]



                      2006




                      • Grimm, C., Ficner, R., Sgraja, T., Haebel, P., Klebe, G. and Reuter, K. (2006). Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyl-transferase- isomerase. Biochem. Biophys. Res. Commun. 351: 695-701. [Abstract]; PDB: [1YY3I]

                      • Hildmann, C., Wegener, D., Riester, D., Hempel, R., Schober, A., Merana, J., Giurato, L., Guccione, S., Nielsen, T.K., Ficner, R. and Schwienhorst, A. (2006). Substrate and inhibitor specificity of class 1 and class 2 histone deacetylases.
                        J. Biotechnol. 124: 258-70. [Abstract]

                      • Dickmanns, A., Ballschmitter, M., Liebl, W. and Ficner, R. (2006). Crystal structure of the novel α-amylase AmyC from Thermotoga maritima. Acta Crystallogr. D62: 262-270. [Abstract]; PDB: [2B5D]



                      2005




                      • Nielsen, T.K., Hildmann, C., Dickmanns, A., Schwienhorst, A. and Ficner, R. (2005). Crystal structure of a bacterial class 2 histone deacetylase.J. Mol. Biol. 354: 107-120. [Abstract]; PDB: [1ZZ0][1ZZ1][1ZZ3]

                      • Andrade, S. L. A., Dickmanns, A., Ficner, R. and Einsle, O. (2005). Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.PNAS. USA, 102: 14994-14999. [Abstract]; PDB: [2B2F][2B2H][2B2I][2B2J]

                      • Andrade, S. L. A., Dickmanns, A., Ficner, R. and Einsle, O. (2005). Expression, Purification and Crystallization of the Ammonium Transporter Amt-1 from Archaeoglobus fulgidus. Acta Crystallogr. F61: 861-863. [Abstract]

                      • Strasser, A., Dickmanns, A., Lührmann, R. and Ficner, R. (2005). Structural basis for m3G-cap mediated nuclear import of spliceosomal UsnRNPs by Snurportin 1. EMBO J. 24: 2235-2243. [Abstract]; PDB: [1XK5]

                      • Dierks, T., Dickmanns, A., Preusser-Kunze, A., Schmidt, B., Mariappan, M., von Figura, K., Ficner, R. and Rudolph, M.G. (2005). Molecular basis for multiple sulfatase deficiency and catalytic mechanism for formylglycine generation of the human formylglycine generating enzyme. Cell 121: 541-552. [Abstract]; PDB: [1Y1E][1Y1F][1Y1G][1Y1H][1Y1I][1Y1J]

                      • Dickmanns, A., Schmidt, B., Rudolph, M.G., Mariappan, M., Dierks, T., von Figura, K. and Ficner, R. (2005). Crystal structure of human pFGE, the paralogue of the Ca-formylglycine generating enzyme. J. Biol. Chem. 280: 15180-15187. [Abstract]; PDB: [1Y4J]

                      • Dickmanns, A. and Ficner, R. (2005). Role of the 5?-cap in the biogenesis of spliceosomal snRNPs. Topics in Current Genetics 12: 179-204.


                      • Arnorsdottir, J., Kristjanson, M.M. and Ficner, R. (2005). Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation. FEBS J. 272: 832-845. [Abstract]; PDB: [1S2N][1S7H]

                      • Stummeyer, K., Dickmanns, A., Mühlenhoff, M., Gerardy-Schahn, R. and Ficner, R. (2005). Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F. Nature Struct. Mol. Biol. 12: 90-96. [Abstract]; PDB: [1V0E][1V0F]



                      2004




                      • Strasser, A., Dickmanns, A., Schmidt, U., Penka, E., Urlaub, H., Sekine, R., Lührmann, R. and Ficner, R. (2004). Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor Snurportin1. Acta Crystallogr. D60: 1628-1631. [Abstract]; PDB: [1XK5]



                      2003




                      • Rudolph, M.G., Kraus, I., Dickmanns, A., Eickmann, M., Garten, W. and Ficner, R. (2003). Crystal Structure of the Borna Disease Virus Nucleoprotein. Structure 11: 1219-1226. [Abstract]; PDB: [1N93][1PP1]

                      • Ingelfinger, D., Gothel, S.F., Marahiel, M.A., Reidt, U., Ficner, R., Lührmann, R. and Achsel, T. (2003).Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH. Nucleic Acids Res. 31: 4791-4796. [Abstract]

                      • Reidt, U., Wahl, M.C., Fasshauer, D., Horowitz, D.S., Lührmann, R. and Ficner, R. (2003).Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide. J. Mol Biol. 331: 45-56. [Abstract]; PDB: [1MZW]



                      2001




                      • Grädler, U., Gerber, H.-D., Goodenough-Lashua, D.M., Garcia, G.A., Ficner, R., Reuter, K., Stubbs, M.T. and Klebe, G. (2001). A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase. J. Mol. Biol. 306: 455-467. [Abstract]; PDB: [1F3E][1ENU]

                      • Maser, E., Xiong, G., Grimm, C., Ficner, R. and Reuter, K. (2001). 3α-Hydroxy­steroid dehydrogenase/ carbonyl reductase from Comomonas testosteroni: biological significance, three-dimensional structure and gene regulation. Chem.-Biol. Interact. 130-132(1-3):707-22. [Abstract]



                      2000




                      • Vidovic, I., Nottrott, S., Hartmuth, K., Lührmann, R. and Ficner, R. (2000). Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment. Mol. Cell 6: 1331-1342. [Abstract]; PDB: [1E7K]

                      • Grimm, C., Maser, E., Möbus, E., Klebe, G., Reuter, K. and Ficner, R. (2000). The crystal structure of 3α-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerisation pattern within the short chain dehydrogenase/ reductase family. J. Biol. Chem. 275: 41333-41339. [Abstract]; PDB: [1FJH][1FK8]

                      • Grimm, C., Klebe, G., Ficner, R. and Reuter, K. (2000). Screening orthologs as an important variable in crystallization: Preliminary X-ray diffraction studies on the tRNA-modifying enzyme S-adenosylmethionine:tRNA-transferase/isomerase. Acta Crystallogr. D56: 484-488. [Abstract]

                      • Reidt, U., Reuter, K., Ingelfinger D., Achsel, T., Lührmann, R. and Ficner, R. (2000). Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin. J. Biol. Chem. 275: 7439-7442. [Abstract]; PDB: [1QOI]



                      1999




                      • Reuter, K., Mofid, M.R., Marahiel, M.A. and Ficner, R. (1999). Crystal structure of the surfactin synthetase activating enzyme Sfp: a prototype of the 4'-phospho- pantetheinyl transferase superfamily. EMBO J. 18: 6823-6831. [Abstract]; PDB: [1QR0]

                      • Reuter, K., Nottrott, S., Fabrizio, P., Lührmann, R. and Ficner, R. (1999). Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. J. Mol. Biol. 294: 515-525. [Abstract]; PDB: [1QGV]

                      • Nottrott, S., Hartmuth, K., Fabrizio, P., Urlaub, H., Vidovic, I., Ficner, R. and Lührmann, R. (1999). Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5?stem-loop of U4 snRNA. EMBO J. 18: 6119-6133. [Abstract]; PDB: [1E7K]

                      • Mofid, M.R., Marahiel, M., Ficner, R. and Reuter, K. (1999). Crystallization and preliminary crystallographic studies of Sfp: a phosphopantetheinyl transferase of modular peptide synthetases. Acta Crystallogr. D55: 1098-1100. [Abstract]; PDB: [1QR0]

                      • Reuter, K. and Ficner, R. (1999). Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein U5-15kD. Acta Crystallogr. D55: 888-890. [Abstract]; PDB: [1QGV]

                      • Grädler, U., Ficner, R., Garcia, G.A., Stubbs, M.T., Klebe, G. and Reuter, K. (1999). Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity. FEBS Lett. 454: 142-146. [Abstract]; PDB: [1EFZ]